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KMID : 1005220080230040239
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Journal of Embryo Transfer
2008 Volume.23 No. 4 p.239 ~ p.243
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Effects of Sugar Type on Viability of Frozen-Thawed Canine Spermatozoa
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Lim Y.H.
Son J.M.
Shin Y.J.
Kim Y.S.
Lee D.S.
Yoon K.Y.
Shin S.T.
Cho J.K.
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Abstract
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This study was conducted to investigate the effects of type of the sugar supplemented to the extender on the vigor, viability and intact acrosomal rates of frozen-thawed dog spermatozoa. The ejaculated semen was diluted with TRIS-citric acid extender containing 200mM TRIS, 73mM citric acid, 6% (v/v) glycerol, 20% (v/v) egg yolk, 1% (v/v) antibiotics (streptomycin/penicillin), 44 mM sugar, which was either glucose, fructose or glucose-fructose combination, and distilled water to make the final volume of 100ml. Extended semen samples were cooled at for an hour, packaged in 0.25ml straws, equilibrated for 10 minutes in liquid nitrogen vapor, and frozen in liquid nitrogen. Samples were thawed by placing straws into water for 120 seconds. After thawing, vigor, viability and intact acrosomal rates of frozen-thawed semen were compared according to type of sugar. No significant differences were observed between glucose and fructose groups. In addition, combination of the 2 sugars also did not show any significant differences in the vigor, viability and intact acrosomal rates. In conclusion, glucose and fructose were equally efficient as sugar supplements for freezing extender.
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KEYWORD
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dog, semen, extender, glucose, fructose, viability
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